Plasmid_Backbone

Part:BBa_K1473005:Design

Designed by: Shan, Zhe   Group: iGEM14_TCU_Taiwan   (2014-09-17)


phagemid pBluescript


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2908
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2914
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2908
    Illegal XhoI site found at 2896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2908
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2908
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2923
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1380
    Illegal SapI site found at 297


Design Notes

Normally, a pBluescript contains EcoRI, PstI, XbaI and SpeI on its MCS. We have removed these sequence to create a linearized form of this phagemid.


Source

Its f1 ori comes from phage M13.

References